Construct: shRNA TRCN0000150816

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015064.2-6577s1c1
DNA Barcode:
GCAAGGACTTTGATACCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ERC1 (23085)
Gene Description:
ELKS/RAB6-interacting/CAST family member 1
Transcript:
RefSeq NM_015064.2 (NON-CURRENT)
Match location:
Position 6577 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150816)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGGACTTTGATACCATTT
Hairpin Sequence:
5'-CCGG-GCAAGGACTTTGATACCATTT-CTCGAG-AAATGGTATCAAAGTCCTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGGACTTTGATACCATTTCTCGAGAAATGGTATCAAAGTCCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGGACTTTGATACCATTTCTCGAGAAATGGTATCAAAGTCCTTGC-3'

Other clones with same target sequence:

(none)