Construct: shRNA TRCN0000150838

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005952.2-114s1c1
DNA Barcode:
GCAAATGCAAAGAGTGCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MT1X (4501)
Gene Description:
metallothionein 1X
Transcript:
RefSeq NM_005952.2 (NON-CURRENT)
Match location:
Position 114 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150838)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAATGCAAAGAGTGCAAAT
Hairpin Sequence:
5'-CCGG-GCAAATGCAAAGAGTGCAAAT-CTCGAG-ATTTGCACTCTTTGCATTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAATGCAAAGAGTGCAAATCTCGAGATTTGCACTCTTTGCATTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAATGCAAAGAGTGCAAATCTCGAGATTTGCACTCTTTGCATTTGC-3'

Other clones with same target sequence:

  1. TRCN0000147402
  2. TRCN0000159156