Construct: shRNA TRCN0000150843

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017632.1-1584s1c1
DNA Barcode:
GAAAGCAACACTGGATGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKN2AIP (55602)
Gene Description:
CDKN2A interacting protein
Transcript:
RefSeq NM_017632.1 (NON-CURRENT)
Match location:
Position 1584 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150843)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGCAACACTGGATGTATT
Hairpin Sequence:
5'-CCGG-GAAAGCAACACTGGATGTATT-CTCGAG-AATACATCCAGTGTTGCTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGCAACACTGGATGTATTCTCGAGAATACATCCAGTGTTGCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGCAACACTGGATGTATTCTCGAGAATACATCCAGTGTTGCTTTC-3'

Other clones with same target sequence:

(none)