Construct: shRNA TRCN0000150892

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015641.2-1193s1c1
DNA Barcode:
GAGATATACGTGATGGTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TES (26136)
Gene Description:
testin LIM domain protein
Transcript:
RefSeq NM_015641.2 (NON-CURRENT)
Match location:
Position 1193 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150892)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATATACGTGATGGTCAAT
Hairpin Sequence:
5'-CCGG-GAGATATACGTGATGGTCAAT-CTCGAG-ATTGACCATCACGTATATCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATATACGTGATGGTCAATCTCGAGATTGACCATCACGTATATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATATACGTGATGGTCAATCTCGAGATTGACCATCACGTATATCTC-3'

Other clones with same target sequence:

  1. TRCN0000353585