Construct: shRNA TRCN0000150903

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030932.2-1743s1c1
DNA Barcode:
GCTCAGTGCTATTCTCTTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DIAPH3 (81624)
Gene Description:
diaphanous related formin 3
Transcript:
RefSeq NM_030932.2 (NON-CURRENT)
Match location:
Position 1743 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150903)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAGTGCTATTCTCTTTAA
Hairpin Sequence:
5'-CCGG-GCTCAGTGCTATTCTCTTTAA-CTCGAG-TTAAAGAGAATAGCACTGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAGTGCTATTCTCTTTAACTCGAGTTAAAGAGAATAGCACTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAGTGCTATTCTCTTTAACTCGAGTTAAAGAGAATAGCACTGAGC-3'

Other clones with same target sequence:

(none)