Construct: shRNA TRCN0000150974

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003176.2-2470s1c1
DNA Barcode:
CCAATCTCAAAGCTGAACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYCP1 (6847)
Gene Description:
synaptonemal complex protein 1
Transcript:
RefSeq NM_003176.2 (NON-CURRENT)
Match location:
Position 2470 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150974)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAATCTCAAAGCTGAACTTT
Hairpin Sequence:
5'-CCGG-CCAATCTCAAAGCTGAACTTT-CTCGAG-AAAGTTCAGCTTTGAGATTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAATCTCAAAGCTGAACTTTCTCGAGAAAGTTCAGCTTTGAGATTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAATCTCAAAGCTGAACTTTCTCGAGAAAGTTCAGCTTTGAGATTGG-3'

Other clones with same target sequence:

(none)