Construct: shRNA TRCN0000150992

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020939.1-910s1c1
DNA Barcode:
CAACATCTATGAGGTGGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CPNE5 (57699)
Gene Description:
copine 5
Transcript:
RefSeq NM_020939.1 (NON-CURRENT)
Match location:
Position 910 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000150992)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACATCTATGAGGTGGTAAA
Hairpin Sequence:
5'-CCGG-CAACATCTATGAGGTGGTAAA-CTCGAG-TTTACCACCTCATAGATGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACATCTATGAGGTGGTAAACTCGAGTTTACCACCTCATAGATGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACATCTATGAGGTGGTAAACTCGAGTTTACCACCTCATAGATGTTG-3'

Other clones with same target sequence:

(none)