Construct: shRNA TRCN0000151035

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021940.2-2922s1c1
DNA Barcode:
GCAGCACAAGTGTAATGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMAP1 (60682)
Gene Description:
small ArfGAP 1
Transcript:
RefSeq NM_021940.2 (NON-CURRENT)
Match location:
Position 2922 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151035)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCACAAGTGTAATGAATA
Hairpin Sequence:
5'-CCGG-GCAGCACAAGTGTAATGAATA-CTCGAG-TATTCATTACACTTGTGCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCACAAGTGTAATGAATACTCGAGTATTCATTACACTTGTGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCACAAGTGTAATGAATACTCGAGTATTCATTACACTTGTGCTGC-3'

Other clones with same target sequence:

(none)