Construct: shRNA TRCN0000151040

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032199.1-6882s1c1
DNA Barcode:
GCCTTCAAAGAGAACCATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARID5B (84159)
Gene Description:
AT-rich interaction domain 5B
Transcript:
RefSeq NM_032199.1 (NON-CURRENT)
Match location:
Position 6882 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151040)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCAAAGAGAACCATTTA
Hairpin Sequence:
5'-CCGG-GCCTTCAAAGAGAACCATTTA-CTCGAG-TAAATGGTTCTCTTTGAAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCAAAGAGAACCATTTACTCGAGTAAATGGTTCTCTTTGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCAAAGAGAACCATTTACTCGAGTAAATGGTTCTCTTTGAAGGC-3'

Other clones with same target sequence:

(none)