Construct: shRNA TRCN0000151045

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173475.1-504s1c1
DNA Barcode:
GCAAAGATTGGAAGAACTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCUN1D3 (123879)
Gene Description:
defective in cullin neddylation 1 domain containing 3
Transcript:
RefSeq NM_173475.1 (NON-CURRENT)
Match location:
Position 504 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151045)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAAGATTGGAAGAACTGTT
Hairpin Sequence:
5'-CCGG-GCAAAGATTGGAAGAACTGTT-CTCGAG-AACAGTTCTTCCAATCTTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAAGATTGGAAGAACTGTTCTCGAGAACAGTTCTTCCAATCTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAAGATTGGAAGAACTGTTCTCGAGAACAGTTCTTCCAATCTTTGC-3'

Other clones with same target sequence:

(none)