Construct: shRNA TRCN0000151193

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032861.2-271s1c1
DNA Barcode:
GCTGTGACATTAGATACTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SERAC1 (84947)
Gene Description:
serine active site containing 1
Transcript:
RefSeq NM_032861.2 (NON-CURRENT)
Match location:
Position 271 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151193)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGACATTAGATACTCAA
Hairpin Sequence:
5'-CCGG-GCTGTGACATTAGATACTCAA-CTCGAG-TTGAGTATCTAATGTCACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGACATTAGATACTCAACTCGAGTTGAGTATCTAATGTCACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGACATTAGATACTCAACTCGAGTTGAGTATCTAATGTCACAGC-3'

Other clones with same target sequence:

(none)