Construct: shRNA TRCN0000151233

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024057.2-111s1c1
DNA Barcode:
GAAGATTATGTGCATGTGGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUP37 (79023)
Gene Description:
nucleoporin 37
Transcript:
RefSeq NM_024057.2 (NON-CURRENT)
Match location:
Position 111 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151233)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGATTATGTGCATGTGGTA
Hairpin Sequence:
5'-CCGG-GAAGATTATGTGCATGTGGTA-CTCGAG-TACCACATGCACATAATCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGATTATGTGCATGTGGTACTCGAGTACCACATGCACATAATCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGATTATGTGCATGTGGTACTCGAGTACCACATGCACATAATCTTC-3'

Other clones with same target sequence:

(none)