Construct: shRNA TRCN0000151254

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033284.1-1376s1c1
DNA Barcode:
GAACAGTGATGGAACACTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBL1Y (90665)
Gene Description:
transducin beta like 1 Y-linked
Transcript:
RefSeq NM_033284.1 (NON-CURRENT)
Match location:
Position 1376 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151254)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACAGTGATGGAACACTATT
Hairpin Sequence:
5'-CCGG-GAACAGTGATGGAACACTATT-CTCGAG-AATAGTGTTCCATCACTGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACAGTGATGGAACACTATTCTCGAGAATAGTGTTCCATCACTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACAGTGATGGAACACTATTCTCGAGAATAGTGTTCCATCACTGTTC-3'

Other clones with same target sequence:

(none)