Construct: shRNA TRCN0000151344

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017632.1-1825s1c1
DNA Barcode:
GAAGATCTAGTACTCCTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKN2AIP (55602)
Gene Description:
CDKN2A interacting protein
Transcript:
RefSeq NM_017632.1 (NON-CURRENT)
Match location:
Position 1825 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151344)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGATCTAGTACTCCTTGAT
Hairpin Sequence:
5'-CCGG-GAAGATCTAGTACTCCTTGAT-CTCGAG-ATCAAGGAGTACTAGATCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGATCTAGTACTCCTTGATCTCGAGATCAAGGAGTACTAGATCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGATCTAGTACTCCTTGATCTCGAGATCAAGGAGTACTAGATCTTC-3'

Other clones with same target sequence:

(none)