Construct: shRNA TRCN0000151354

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024744.12-2918s1c1
DNA Barcode:
GTCTTAGAAAGGAAGGTGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CARF (79800)
Gene Description:
calcium responsive transcription factor
Transcript:
RefSeq NM_024744.12 (NON-CURRENT)
Match location:
Position 2918 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151354)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTTAGAAAGGAAGGTGAAT
Hairpin Sequence:
5'-CCGG-GTCTTAGAAAGGAAGGTGAAT-CTCGAG-ATTCACCTTCCTTTCTAAGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTTAGAAAGGAAGGTGAATCTCGAGATTCACCTTCCTTTCTAAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTTAGAAAGGAAGGTGAATCTCGAGATTCACCTTCCTTTCTAAGAC-3'

Other clones with same target sequence:

(none)