Construct: shRNA TRCN0000151400

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198137.1-858s1c1
DNA Barcode:
CTTTACCATCTTCATCACCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CATSPER4 (378807)
Gene Description:
cation channel sperm associated 4
Transcript:
RefSeq NM_198137.1 (CURRENT)
Match location:
Position 858 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151400)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTACCATCTTCATCACCAT
Hairpin Sequence:
5'-CCGG-CTTTACCATCTTCATCACCAT-CTCGAG-ATGGTGATGAAGATGGTAAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTACCATCTTCATCACCATCTCGAGATGGTGATGAAGATGGTAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTACCATCTTCATCACCATCTCGAGATGGTGATGAAGATGGTAAAG-3'

Other clones with same target sequence:

(none)