Construct: shRNA TRCN0000151409

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005952.2-279s1c1
DNA Barcode:
GCAACCTATATAAACCTGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MT1X (4501)
Gene Description:
metallothionein 1X
Transcript:
RefSeq NM_005952.2 (NON-CURRENT)
Match location:
Position 279 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151409)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACCTATATAAACCTGGAT
Hairpin Sequence:
5'-CCGG-GCAACCTATATAAACCTGGAT-CTCGAG-ATCCAGGTTTATATAGGTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACCTATATAAACCTGGATCTCGAGATCCAGGTTTATATAGGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACCTATATAAACCTGGATCTCGAGATCCAGGTTTATATAGGTTGC-3'

Other clones with same target sequence:

(none)