Construct: shRNA TRCN0000151418

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145052.1-632s1c1
DNA Barcode:
GTACAAGTATGAAGGAGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UPRT (139596)
Gene Description:
uracil phosphoribosyltransferase homolog
Transcript:
RefSeq NM_145052.1 (NON-CURRENT)
Match location:
Position 632 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151418)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACAAGTATGAAGGAGTGAA
Hairpin Sequence:
5'-CCGG-GTACAAGTATGAAGGAGTGAA-CTCGAG-TTCACTCCTTCATACTTGTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACAAGTATGAAGGAGTGAACTCGAGTTCACTCCTTCATACTTGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACAAGTATGAAGGAGTGAACTCGAGTTCACTCCTTCATACTTGTAC-3'

Other clones with same target sequence:

(none)