Construct: shRNA TRCN0000151450

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015512.3-806s1c1
DNA Barcode:
CCACTGAAGGTATTTGACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNAH1 (25981)
Gene Description:
dynein axonemal heavy chain 1
Transcript:
RefSeq NM_015512.3 (NON-CURRENT)
Match location:
Position 806 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151450)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTGAAGGTATTTGACAAT
Hairpin Sequence:
5'-CCGG-CCACTGAAGGTATTTGACAAT-CTCGAG-ATTGTCAAATACCTTCAGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTGAAGGTATTTGACAATCTCGAGATTGTCAAATACCTTCAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTGAAGGTATTTGACAATCTCGAGATTGTCAAATACCTTCAGTGG-3'

Other clones with same target sequence:

(none)