Construct: shRNA TRCN0000151452

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016070.2-746s1c1
DNA Barcode:
CAAAGCTCAGTATCATGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRPS23 (51649)
Gene Description:
mitochondrial ribosomal protein S23
Transcript:
RefSeq NM_016070.2 (NON-CURRENT)
Match location:
Position 746 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151452)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGCTCAGTATCATGGTTT
Hairpin Sequence:
5'-CCGG-CAAAGCTCAGTATCATGGTTT-CTCGAG-AAACCATGATACTGAGCTTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGCTCAGTATCATGGTTTCTCGAGAAACCATGATACTGAGCTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGCTCAGTATCATGGTTTCTCGAGAAACCATGATACTGAGCTTTG-3'

Other clones with same target sequence:

(none)