Construct: shRNA TRCN0000151477

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013321.1-590s1c1
DNA Barcode:
CAGAACAAGTTAAAGGAGTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNX8 (29886)
Gene Description:
sorting nexin 8
Transcript:
RefSeq NM_013321.1 (NON-CURRENT)
Match location:
Position 590 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151477)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAACAAGTTAAAGGAGTCA
Hairpin Sequence:
5'-CCGG-CAGAACAAGTTAAAGGAGTCA-CTCGAG-TGACTCCTTTAACTTGTTCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAACAAGTTAAAGGAGTCACTCGAGTGACTCCTTTAACTTGTTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAACAAGTTAAAGGAGTCACTCGAGTGACTCCTTTAACTTGTTCTG-3'

Other clones with same target sequence:

(none)