Construct: shRNA TRCN0000151480

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014897.1-1127s1c1
DNA Barcode:
CGCATGCAGATTTGTGATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF652 (22834)
Gene Description:
zinc finger protein 652
Transcript:
RefSeq NM_014897.1 (NON-CURRENT)
Match location:
Position 1127 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151480)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCATGCAGATTTGTGATAAA
Hairpin Sequence:
5'-CCGG-CGCATGCAGATTTGTGATAAA-CTCGAG-TTTATCACAAATCTGCATGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCATGCAGATTTGTGATAAACTCGAGTTTATCACAAATCTGCATGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCATGCAGATTTGTGATAAACTCGAGTTTATCACAAATCTGCATGCG-3'

Other clones with same target sequence:

  1. TRCN0000279635