Construct: shRNA TRCN0000151493

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032318.1-452s1c1
DNA Barcode:
CTCATGAATGGTCTCATTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MFSD14C (84278)
Gene Description:
major facilitator superfamily domain containing 14C
Transcript:
RefSeq NM_032318.1 (NON-CURRENT)
Match location:
Position 452 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151493)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCATGAATGGTCTCATTCAA
Hairpin Sequence:
5'-CCGG-CTCATGAATGGTCTCATTCAA-CTCGAG-TTGAATGAGACCATTCATGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCATGAATGGTCTCATTCAACTCGAGTTGAATGAGACCATTCATGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCATGAATGGTCTCATTCAACTCGAGTTGAATGAGACCATTCATGAG-3'

Other clones with same target sequence:

(none)