Construct: shRNA TRCN0000151545

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015512.3-6934s1c1
DNA Barcode:
GCGCAACTTTATCTTCTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNAH1 (25981)
Gene Description:
dynein axonemal heavy chain 1
Transcript:
RefSeq NM_015512.3 (NON-CURRENT)
Match location:
Position 6934 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151545)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCAACTTTATCTTCTTCAT
Hairpin Sequence:
5'-CCGG-GCGCAACTTTATCTTCTTCAT-CTCGAG-ATGAAGAAGATAAAGTTGCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCAACTTTATCTTCTTCATCTCGAGATGAAGAAGATAAAGTTGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCAACTTTATCTTCTTCATCTCGAGATGAAGAAGATAAAGTTGCGC-3'

Other clones with same target sequence:

(none)