Construct: shRNA TRCN0000151630

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032730.2-428s1c1
DNA Barcode:
GAAGAATGAAGTGCTTCGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RTN4IP1 (84816)
Gene Description:
reticulon 4 interacting protein 1
Transcript:
RefSeq NM_032730.2 (NON-CURRENT)
Match location:
Position 428 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151630)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAATGAAGTGCTTCGATT
Hairpin Sequence:
5'-CCGG-GAAGAATGAAGTGCTTCGATT-CTCGAG-AATCGAAGCACTTCATTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAATGAAGTGCTTCGATTCTCGAGAATCGAAGCACTTCATTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAATGAAGTGCTTCGATTCTCGAGAATCGAAGCACTTCATTCTTC-3'

Other clones with same target sequence:

(none)