Construct: shRNA TRCN0000151639

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003928.1-1030s1c1
DNA Barcode:
CATAACGCAGTATTCTGAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RTL8C (8933)
Gene Description:
retrotransposon Gag like 8C
Transcript:
RefSeq NM_003928.1 (NON-CURRENT)
Match location:
Position 1030 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151639)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATAACGCAGTATTCTGAGTT
Hairpin Sequence:
5'-CCGG-CATAACGCAGTATTCTGAGTT-CTCGAG-AACTCAGAATACTGCGTTATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATAACGCAGTATTCTGAGTTCTCGAGAACTCAGAATACTGCGTTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATAACGCAGTATTCTGAGTTCTCGAGAACTCAGAATACTGCGTTATG-3'

Other clones with same target sequence:

(none)