Construct: shRNA TRCN0000151640

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004161.3-322s1c1
DNA Barcode:
CTTCTTAGGTTTGCAGATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB1A (5861)
Gene Description:
RAB1A, member RAS oncogene family
Transcript:
RefSeq NM_004161.3 (NON-CURRENT)
Match location:
Position 322 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151640)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTTAGGTTTGCAGATGAT
Hairpin Sequence:
5'-CCGG-CTTCTTAGGTTTGCAGATGAT-CTCGAG-ATCATCTGCAAACCTAAGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTTAGGTTTGCAGATGATCTCGAGATCATCTGCAAACCTAAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTTAGGTTTGCAGATGATCTCGAGATCATCTGCAAACCTAAGAAG-3'

Other clones with same target sequence:

  1. TRCN0000297879