Construct: shRNA TRCN0000151710

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207438.1-785s1c1
DNA Barcode:
CCAGAGACATAGTTGATTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNAH10 (196385)
Gene Description:
dynein axonemal heavy chain 10
Transcript:
RefSeq NM_207438.1 (NON-CURRENT)
Match location:
Position 785 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151710)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGACATAGTTGATTCTT
Hairpin Sequence:
5'-CCGG-CCAGAGACATAGTTGATTCTT-CTCGAG-AAGAATCAACTATGTCTCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGACATAGTTGATTCTTCTCGAGAAGAATCAACTATGTCTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGACATAGTTGATTCTTCTCGAGAAGAATCAACTATGTCTCTGG-3'

Other clones with same target sequence:

(none)