Construct: shRNA TRCN0000151741

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021937.2-250s1c1
DNA Barcode:
CCAGATCATTGATCTGATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EEFSEC (60678)
Gene Description:
eukaryotic elongation factor, selenocysteine-tRNA specific
Transcript:
RefSeq NM_021937.2 (NON-CURRENT)
Match location:
Position 250 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151741)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATCATTGATCTGATGAT
Hairpin Sequence:
5'-CCGG-CCAGATCATTGATCTGATGAT-CTCGAG-ATCATCAGATCAATGATCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATCATTGATCTGATGATCTCGAGATCATCAGATCAATGATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATCATTGATCTGATGATCTCGAGATCATCAGATCAATGATCTGG-3'

Other clones with same target sequence:

(none)