Construct: shRNA TRCN0000151751

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175620.1-92s1c1
DNA Barcode:
CTGCAAATGCAAAGAGTACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MT1DP (326343)
Gene Description:
metallothionein 1D, pseudogene
Transcript:
RefSeq NM_175620.1 (NON-CURRENT)
Match location:
Position 92 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151751)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCAAATGCAAAGAGTACAA
Hairpin Sequence:
5'-CCGG-CTGCAAATGCAAAGAGTACAA-CTCGAG-TTGTACTCTTTGCATTTGCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCAAATGCAAAGAGTACAACTCGAGTTGTACTCTTTGCATTTGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCAAATGCAAAGAGTACAACTCGAGTTGTACTCTTTGCATTTGCAG-3'

Other clones with same target sequence:

(none)