Construct: shRNA TRCN0000151754

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001003795.1-2905s1c1
DNA Barcode:
CGCAAAGATTCTTTCCATGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GTF2IRD2B (389524)
Gene Description:
GTF2I repeat domain containing 2B
Transcript:
RefSeq NM_001003795.1 (NON-CURRENT)
Match location:
Position 2905 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151754)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAAAGATTCTTTCCATGTT
Hairpin Sequence:
5'-CCGG-CGCAAAGATTCTTTCCATGTT-CTCGAG-AACATGGAAAGAATCTTTGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAAAGATTCTTTCCATGTTCTCGAGAACATGGAAAGAATCTTTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAAAGATTCTTTCCATGTTCTCGAGAACATGGAAAGAATCTTTGCG-3'

Other clones with same target sequence:

(none)