Construct: shRNA TRCN0000151759

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004751.1-500s1c1
DNA Barcode:
CCAGTACTGTAGGAATATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GCNT3 (9245)
Gene Description:
glucosaminyl (N-acetyl) transferase 3, mucin type
Transcript:
RefSeq NM_004751.1 (NON-CURRENT)
Match location:
Position 500 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151759)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTACTGTAGGAATATCTT
Hairpin Sequence:
5'-CCGG-CCAGTACTGTAGGAATATCTT-CTCGAG-AAGATATTCCTACAGTACTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTACTGTAGGAATATCTTCTCGAGAAGATATTCCTACAGTACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTACTGTAGGAATATCTTCTCGAGAAGATATTCCTACAGTACTGG-3'

Other clones with same target sequence:

  1. TRCN0000279666