Construct: shRNA TRCN0000151808

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006172.1-633s1c1
DNA Barcode:
CTGCATTTGTGTCATCTTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPPA (4878)
Gene Description:
natriuretic peptide A
Transcript:
RefSeq NM_006172.1 (NON-CURRENT)
Match location:
Position 633 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151808)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCATTTGTGTCATCTTGTT
Hairpin Sequence:
5'-CCGG-CTGCATTTGTGTCATCTTGTT-CTCGAG-AACAAGATGACACAAATGCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCATTTGTGTCATCTTGTTCTCGAGAACAAGATGACACAAATGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCATTTGTGTCATCTTGTTCTCGAGAACAAGATGACACAAATGCAG-3'

Other clones with same target sequence:

(none)