Construct: shRNA TRCN0000151985

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003285.1-4521s1c1
DNA Barcode:
CCTTCCAGGTATTTGTGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNR (7143)
Gene Description:
tenascin R
Transcript:
RefSeq NM_003285.1 (NON-CURRENT)
Match location:
Position 4521 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000151985)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCCAGGTATTTGTGATTT
Hairpin Sequence:
5'-CCGG-CCTTCCAGGTATTTGTGATTT-CTCGAG-AAATCACAAATACCTGGAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCCAGGTATTTGTGATTTCTCGAGAAATCACAAATACCTGGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCCAGGTATTTGTGATTTCTCGAGAAATCACAAATACCTGGAAGG-3'

Other clones with same target sequence:

(none)