Construct: shRNA TRCN0000152019

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018840.2-443s1c1
DNA Barcode:
CTTCAGCAATTACCTACAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB5IF (55969)
Gene Description:
RAB5 interacting factor
Transcript:
RefSeq NM_018840.2 (NON-CURRENT)
Match location:
Position 443 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000152019)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCAGCAATTACCTACAGAT
Hairpin Sequence:
5'-CCGG-CTTCAGCAATTACCTACAGAT-CTCGAG-ATCTGTAGGTAATTGCTGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCAGCAATTACCTACAGATCTCGAGATCTGTAGGTAATTGCTGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCAGCAATTACCTACAGATCTCGAGATCTGTAGGTAATTGCTGAAG-3'

Other clones with same target sequence:

  1. TRCN0000275078