Construct: shRNA TRCN0000152025

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032445.1-3600s1c1
DNA Barcode:
CCATCAAATTACAACAGCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MEGF11 (84465)
Gene Description:
multiple EGF like domains 11
Transcript:
RefSeq NM_032445.1 (NON-CURRENT)
Match location:
Position 3600 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000152025)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCAAATTACAACAGCCAA
Hairpin Sequence:
5'-CCGG-CCATCAAATTACAACAGCCAA-CTCGAG-TTGGCTGTTGTAATTTGATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCAAATTACAACAGCCAACTCGAGTTGGCTGTTGTAATTTGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCAAATTACAACAGCCAACTCGAGTTGGCTGTTGTAATTTGATGG-3'

Other clones with same target sequence:

(none)