Construct: shRNA TRCN0000152109

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016316.1-4007s1c1
DNA Barcode:
CATAAGTGCTTGTGAGGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
REV1 (51455)
Gene Description:
REV1 DNA directed polymerase
Transcript:
RefSeq NM_016316.1 (NON-CURRENT)
Match location:
Position 4007 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000152109)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATAAGTGCTTGTGAGGTATT
Hairpin Sequence:
5'-CCGG-CATAAGTGCTTGTGAGGTATT-CTCGAG-AATACCTCACAAGCACTTATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATAAGTGCTTGTGAGGTATTCTCGAGAATACCTCACAAGCACTTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATAAGTGCTTGTGAGGTATTCTCGAGAATACCTCACAAGCACTTATG-3'

Other clones with same target sequence:

(none)