Construct: shRNA TRCN0000152155

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145658.1-604s1c1
DNA Barcode:
CAAGAATGTTGCCAGTTGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPESP1 (246777)
Gene Description:
sperm equatorial segment protein 1
Transcript:
RefSeq NM_145658.1 (NON-CURRENT)
Match location:
Position 604 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000152155)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGAATGTTGCCAGTTGTTA
Hairpin Sequence:
5'-CCGG-CAAGAATGTTGCCAGTTGTTA-CTCGAG-TAACAACTGGCAACATTCTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGAATGTTGCCAGTTGTTACTCGAGTAACAACTGGCAACATTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGAATGTTGCCAGTTGTTACTCGAGTAACAACTGGCAACATTCTTG-3'

Other clones with same target sequence:

(none)