Construct: shRNA TRCN0000152165

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004713.2-3357s1c1
DNA Barcode:
CAAAGATGAAAGCACCATGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEMF (9147)
Gene Description:
nuclear export mediator factor
Transcript:
RefSeq NM_004713.2 (NON-CURRENT)
Match location:
Position 3357 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000152165)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGATGAAAGCACCATGTA
Hairpin Sequence:
5'-CCGG-CAAAGATGAAAGCACCATGTA-CTCGAG-TACATGGTGCTTTCATCTTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGATGAAAGCACCATGTACTCGAGTACATGGTGCTTTCATCTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGATGAAAGCACCATGTACTCGAGTACATGGTGCTTTCATCTTTG-3'

Other clones with same target sequence:

(none)