Construct: shRNA TRCN0000152254

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031442.2-652s1c1
DNA Barcode:
CCTTTACCCAATCAAGTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM47 (83604)
Gene Description:
transmembrane protein 47
Transcript:
RefSeq NM_031442.2 (NON-CURRENT)
Match location:
Position 652 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000152254)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTACCCAATCAAGTTCAT
Hairpin Sequence:
5'-CCGG-CCTTTACCCAATCAAGTTCAT-CTCGAG-ATGAACTTGATTGGGTAAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTACCCAATCAAGTTCATCTCGAGATGAACTTGATTGGGTAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTACCCAATCAAGTTCATCTCGAGATGAACTTGATTGGGTAAAGG-3'

Other clones with same target sequence:

  1. TRCN0000330934