Construct: shRNA TRCN0000152367

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020336.1-1212s1c1
DNA Barcode:
CCGCAAGAATTGAATCAGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RALGAPB (57148)
Gene Description:
Ral GTPase activating protein non-catalytic beta subunit
Transcript:
RefSeq NM_020336.1 (NON-CURRENT)
Match location:
Position 1212 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000152367)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCAAGAATTGAATCAGTAT
Hairpin Sequence:
5'-CCGG-CCGCAAGAATTGAATCAGTAT-CTCGAG-ATACTGATTCAATTCTTGCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCAAGAATTGAATCAGTATCTCGAGATACTGATTCAATTCTTGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCAAGAATTGAATCAGTATCTCGAGATACTGATTCAATTCTTGCGG-3'

Other clones with same target sequence:

  1. TRCN0000318984