Construct: shRNA TRCN0000152584
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_032445.1-1275s1c1
- DNA Barcode:
- GTCCATCTGTAGCTGTAACAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MEGF11 (84465)
- Gene Description:
- multiple EGF like domains 11
- Transcript:
- RefSeq NM_032445.1 (NON-CURRENT)
- Match location:
- Position 1275 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000152584)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GTCCATCTGTAGCTGTAACAA
- Hairpin Sequence:
-
5'-CCGG-GTCCATCTGTAGCTGTAACAA-CTCGAG-TTGTTACAGCTACAGATGGAC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGTCCATCTGTAGCTGTAACAACTCGAGTTGTTACAGCTACAGATGGACTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGTCCATCTGTAGCTGTAACAACTCGAGTTGTTACAGCTACAGATGGAC-3'
Other clones with same target sequence:
(none)
