Construct: shRNA TRCN0000152630

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024894.1-2853s1c1
DNA Barcode:
GCACACTTTCAAACACCAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NOL10 (79954)
Gene Description:
nucleolar protein 10
Transcript:
RefSeq NM_024894.1 (NON-CURRENT)
Match location:
Position 2853 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000152630)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACACTTTCAAACACCAGAT
Hairpin Sequence:
5'-CCGG-GCACACTTTCAAACACCAGAT-CTCGAG-ATCTGGTGTTTGAAAGTGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACACTTTCAAACACCAGATCTCGAGATCTGGTGTTTGAAAGTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACACTTTCAAACACCAGATCTCGAGATCTGGTGTTTGAAAGTGTGC-3'

Other clones with same target sequence:

(none)