Construct: shRNA TRCN0000152638

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139072.2-1221s1c1
DNA Barcode:
GCGAGCTGTATTGATGCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNER (92737)
Gene Description:
delta/notch like EGF repeat containing
Transcript:
RefSeq NM_139072.2 (NON-CURRENT)
Match location:
Position 1221 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000152638)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAGCTGTATTGATGCAAAT
Hairpin Sequence:
5'-CCGG-GCGAGCTGTATTGATGCAAAT-CTCGAG-ATTTGCATCAATACAGCTCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAGCTGTATTGATGCAAATCTCGAGATTTGCATCAATACAGCTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAGCTGTATTGATGCAAATCTCGAGATTTGCATCAATACAGCTCGC-3'

Other clones with same target sequence:

  1. TRCN0000099741