Construct: shRNA TRCN0000152661

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014878.3-1782s1c1
DNA Barcode:
GTGTAAATCGAGGTGCCATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PUM3 (9933)
Gene Description:
pumilio RNA binding family member 3
Transcript:
RefSeq NM_014878.3 (NON-CURRENT)
Match location:
Position 1782 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000152661)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTAAATCGAGGTGCCATTA
Hairpin Sequence:
5'-CCGG-GTGTAAATCGAGGTGCCATTA-CTCGAG-TAATGGCACCTCGATTTACAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTAAATCGAGGTGCCATTACTCGAGTAATGGCACCTCGATTTACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTAAATCGAGGTGCCATTACTCGAGTAATGGCACCTCGATTTACAC-3'

Other clones with same target sequence:

  1. TRCN0000280606