Construct: shRNA TRCN0000152855

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002783.1-542s1c1
DNA Barcode:
GCTGTGATCTTAACCTGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSG7 (5676)
Gene Description:
pregnancy specific beta-1-glycoprotein 7 (gene/pseudogene)
Transcript:
RefSeq NM_002783.1 (NON-CURRENT)
Match location:
Position 542 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000152855)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGATCTTAACCTGTGAT
Hairpin Sequence:
5'-CCGG-GCTGTGATCTTAACCTGTGAT-CTCGAG-ATCACAGGTTAAGATCACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGATCTTAACCTGTGATCTCGAGATCACAGGTTAAGATCACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGATCTTAACCTGTGATCTCGAGATCACAGGTTAAGATCACAGC-3'

Other clones with same target sequence:

  1. TRCN0000146542