Construct: shRNA TRCN0000152922

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014314.2-4152s1c1
DNA Barcode:
CCAGAGAAACTTGCCAGTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDX58 (23586)
Gene Description:
DExD/H-box helicase 58
Transcript:
RefSeq NM_014314.2 (NON-CURRENT)
Match location:
Position 4152 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000152922)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGAAACTTGCCAGTTAT
Hairpin Sequence:
5'-CCGG-CCAGAGAAACTTGCCAGTTAT-CTCGAG-ATAACTGGCAAGTTTCTCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGAAACTTGCCAGTTATCTCGAGATAACTGGCAAGTTTCTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGAAACTTGCCAGTTATCTCGAGATAACTGGCAAGTTTCTCTGG-3'

Other clones with same target sequence:

(none)