Construct: shRNA TRCN0000152962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198547.1-2057s1c1
DNA Barcode:
GTGTTGAACCACATGCTTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MROH7 (374977)
Gene Description:
maestro heat like repeat family member 7
Transcript:
RefSeq NM_198547.1 (NON-CURRENT)
Match location:
Position 2057 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000152962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTTGAACCACATGCTTCTA
Hairpin Sequence:
5'-CCGG-GTGTTGAACCACATGCTTCTA-CTCGAG-TAGAAGCATGTGGTTCAACAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTTGAACCACATGCTTCTACTCGAGTAGAAGCATGTGGTTCAACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTTGAACCACATGCTTCTACTCGAGTAGAAGCATGTGGTTCAACAC-3'

Other clones with same target sequence:

(none)