Construct: shRNA TRCN0000152972

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015157.1-2748s1c1
DNA Barcode:
GAGACTGAGACAAAGCTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHLDB1 (23187)
Gene Description:
pleckstrin homology like domain family B member 1
Transcript:
RefSeq NM_015157.1 (NON-CURRENT)
Match location:
Position 2748 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000152972)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACTGAGACAAAGCTCTTT
Hairpin Sequence:
5'-CCGG-GAGACTGAGACAAAGCTCTTT-CTCGAG-AAAGAGCTTTGTCTCAGTCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACTGAGACAAAGCTCTTTCTCGAGAAAGAGCTTTGTCTCAGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACTGAGACAAAGCTCTTTCTCGAGAAAGAGCTTTGTCTCAGTCTC-3'

Other clones with same target sequence:

(none)