Construct: shRNA TRCN0000152985

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033082.1-340s1c1
DNA Barcode:
GCTGAACGATTCAATGTACCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SARNP (84324)
Gene Description:
SAP domain containing ribonucleoprotein
Transcript:
RefSeq NM_033082.1 (NON-CURRENT)
Match location:
Position 340 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000152985)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAACGATTCAATGTACCT
Hairpin Sequence:
5'-CCGG-GCTGAACGATTCAATGTACCT-CTCGAG-AGGTACATTGAATCGTTCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAACGATTCAATGTACCTCTCGAGAGGTACATTGAATCGTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAACGATTCAATGTACCTCTCGAGAGGTACATTGAATCGTTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000120011
  2. TRCN0000345385